FastQCFastQC Report
Sun 19 Mar 2023
SRR4895823_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895823_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345819
Sequences flagged as poor quality0
Sequence length151
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGG100.00721097144.174099
ACTGACT100.00721097144.174097
CGTAGAG151.2576078E-4144.174098
ACGTCAT400.0144.174097
CGACTGA100.00721097144.174098
AGGACAC346600.0143.841322
GGACACT131750.0143.626943
GACACTA38900.0143.432834
AAGGACA347600.0143.42751
GACACTG43700.0143.184344
GGACACA99750.0142.945533
GGACACG107000.0142.826683
GACACGT36750.0142.604844
GACACGA34300.0142.28264
GACACTT48500.0142.093234
ACACTAG12700.0141.903645
GACACAT36000.0141.370714
GACACAG34550.0140.418474
GACACGG35950.0139.963174
ACACTGA11800.0139.897745
Produced by FastQC (version 0.11.5)