FastQCFastQC Report
Sun 19 Mar 2023
SRR4895823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345819
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51891.5004959241684233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG46811.35359826961503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA12700.36724413638348385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9040.26140842463832237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8350.2414557904568575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7930.22931070878118323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGAGTCTGGAAATAACT4120.11913746786613806No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA4030.11653495036420787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3640.10525737452251033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT137300.0117.655341
GGTATCA139750.0115.7834858
GTATCAA140850.0115.239539
GTGGTAT140400.0114.472936
AGCAGTG140700.0114.45152
AGTGGTA140600.0114.429765
TGGTATC141000.0114.140057
CAGTGGT140850.0114.123724
GCAGTGG143700.0111.708933
AGTTCCG604.9424596E-448.329575145
AGGACAC2500.043.5029072
CGAATGA750.001482609738.663662145
CTCCACT4900.038.466396145
AGGGGTA1209.5413925E-636.2524225
AGAGGTA1406.5895074E-736.2524225
GAGGTAT1501.1317334E-633.8307046
GGGTATC1555.4930824E-528.0623327
AGTATCA1306.477255E-427.8824488
GGGATAC1300.026.77101925-29
CGGTCTG1100.0096666726.361588145
Produced by FastQC (version 0.11.5)