FastQCFastQC Report
Sun 19 Mar 2023
SRR4895822_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895822_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444475
Sequences flagged as poor quality0
Sequence length151
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAG100.007210387144.182598
GATCCGA253.835339E-8144.182575
AGAGATC445950.0143.697622
GAGATCA133400.0143.642183
GAGATCG133750.0143.53583
AAGAGAT446900.0143.392141
AGATCAG41600.0142.969514
AGATCGA43450.0142.855244
GATCGAG14500.0142.691045
AGATCTG55750.0142.501534
AGATCAT51050.0142.487994
AGATCGT56000.0142.38034
GATCGTA15700.0142.345875
GATCTAG15400.0142.310075
AGATCAA40850.0142.064854
GAGATCT171100.0142.033743
GATCTGA13000.0141.409845
GATCAGT14950.0141.289285
GATCAAT14850.0141.26985
GATCGTG16900.0141.196565
Produced by FastQC (version 0.11.5)