FastQCFastQC Report
Sun 19 Mar 2023
SRR4895822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444475
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77871.7519545531244727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG56991.2821868496540862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10230.23015917655661175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6460.14534000787445864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6090.1370155801788627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT5120.11519208054446257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT171400.0119.841281
AGTGGTA173400.0117.609515
GGTATCA174350.0117.467678
GTATCAA175250.0116.988529
GTGGTAT174450.0116.818526
AGCAGTG175950.0116.6054842
TGGTATC175400.0116.268487
CAGTGGT175750.0116.078184
GCAGTGG176900.0115.3645553
ACGTGTT1600.063.442272145
CCGCAAG2850.053.425076145
ATAGGCC657.3294487E-444.618744145
GTGATAT2800.036.248656
AGGGGTA1251.2638324E-534.7987025
GAGGTAT1301.653143E-533.4602936
CGGTCTG1102.4329072E-432.957027145
CAGGGGT1402.7439704E-531.070274
TCGTCGG304.3995155E-529.0015390-94
GATATCA3800.026.7095328
TGATATC3900.026.0246737
Produced by FastQC (version 0.11.5)