FastQCFastQC Report
Sun 19 Mar 2023
SRR4895821_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895821_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645598
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG110681.714379536491749No Hit
AACTCACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG19920.3085511417321615No Hit
AACTCACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT15290.23683468660064003No Hit
AACTCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC9610.14885424056456184No Hit
AACTCACCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG8870.13739199935563617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC647700.0143.72281
ACTCACC648100.0143.634082
CTCACCG206200.0143.257373
TCACCGT73700.0142.636184
CTCACCT207900.0142.398073
TCACCGA60700.0142.182164
CTCACCA216850.0142.173263
TCACCTG69700.0142.132574
TCACCAT98500.0141.712694
CACCATG51950.0141.425675
TCACCTA62700.0141.326634
TCACCAG63250.0141.009644
TCACCGG71450.0140.77054
ACCATGT37150.0139.737616
CACCGTA22900.0139.47875
CACCTGA19400.0139.369965
CACCGAT21650.0139.206025
CACCTAG22150.0138.993275
ACCGTAT6400.0138.568596
CACCAGT21550.0138.513695
Produced by FastQC (version 0.11.5)