FastQCFastQC Report
Sun 19 Mar 2023
SRR4895821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG280194.340007249093089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58610.9078404827772082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA48500.7512414846390478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39920.6183414446760988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21090.32667387445438184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACGATGGCTCACGCCT7230.11198919451423331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG7080.1096657672421538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT255550.076.689881
AGCAGTG265150.073.924712
AGTGGTA264050.073.903125
GTGGTAT265850.073.478886
CAGTGGT266450.073.237454
GTATCAA268650.073.160559
TGGTATC267500.073.134077
GGTATCA268500.073.104748
GCAGTGG268800.072.651123
CTCACCG1109.9344106E-846.146693
CCGACGC700.001056809741.425007145
AAATATA17750.034.306908145
CTCGAAT950.00472268530.5307856
GCCCCAA6500.028.997507145
TGCGCAA1000.006075435328.997507145
ATCTAGG1255.1544356E-428.997505145
CGGTCTG1751.8189894E-1228.997505145
CGGAGCC2303.486639E-828.367125145
CCGCAAG4450.026.065174145
AGCGCAT1409.99576E-425.890629145
Produced by FastQC (version 0.11.5)