FastQCFastQC Report
Sun 19 Mar 2023
SRR4895820_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895820_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651881
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT55990.8588990935462146No Hit
TTCACGCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8180.12548302527608568No Hit
TTCACGCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT8180.12548302527608568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA652800.0144.02192
TTCACGC652800.0144.02191
CACGCAT245700.0143.87963
CACGCAG210700.0143.615723
ACGCAGT73750.0143.54734
ACGCATA68400.0143.388354
ACGCAAT71050.0143.31834
ACGCATG94550.0143.316534
ACGCATT80450.0143.245764
CGCAGTA23400.0142.999045
CACGCAA186650.0142.995423
CGCATTG29050.0142.742315
CGCATTA22750.0142.329855
CGCATAT22950.0142.03225
ACGCAGA64900.0142.009434
CGCATAG21800.0141.916155
CGCAATG23550.0141.782015
CGCATAA22750.0141.695865
CGCAAGT21800.0141.585345
ACGCAAG64050.0141.07924
Produced by FastQC (version 0.11.5)