FastQCFastQC Report
Sun 19 Mar 2023
SRR4895820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651881
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG157952.4229882447870086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53090.814412446443446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27790.42630480102963575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12080.18530989551774021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT9570.1468059354391369No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8840.13560757254775027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7640.11719930478108734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT183200.088.218211
GGTATCA189700.085.105948
GTGGTAT189250.085.040166
AGCAGTG189800.084.997762
GTATCAA190900.084.608939
TGGTATC191050.084.314847
AGTGGTA191350.084.075455
CAGTGGT192950.083.534964
GCAGTGG197000.081.707213
CGGTCTG1650.057.11713145
TCACGCA1500.053.171032
TTCACGC1550.051.4558331
CGGAGCC3800.028.616375145
CACGCAT1555.494456E-528.0668163
CCGCAAG6750.026.849934145
CGATAGG459.120122E-725.77712235-39
TACGCAC2100.025.54579550-54
CATGCGT6550.024.349405145
CATGGGG47050.023.94960225-29
CGACCTA2400.022.95669245-49
Produced by FastQC (version 0.11.5)