FastQCFastQC Report
Sun 19 Mar 2023
SRR4895819_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895819_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127657
Sequences flagged as poor quality0
Sequence length151
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA1135550.0143.20062
GTGGTAG395400.0142.544433
GTGGTAT404350.0141.97493
GTGGTAA328500.0141.745563
TGGTGGT1152650.0141.076171
TGGTAGT147350.0139.750984
TGGTAAG116650.0139.38264
TGGTAGA112500.0138.756134
TGGTATG142750.0138.294254
TGGTATA123650.0137.672974
TGGTAGG140300.0137.574364
TGGTAAT122600.0137.087744
GGTATAG43900.0134.18515
GGTAGTA44500.0133.834125
GGTAATG45200.0133.516165
TAGTCGA1200.0132.186847
TGGTAAA100000.0130.504474
TGGTATT153700.0130.083684
GGTATTG53950.0129.369145
GTGGTAC17650.0129.088973
Produced by FastQC (version 0.11.5)