FastQCFastQC Report
Sun 19 Mar 2023
SRR4895819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127657
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121001.0730213176524424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79340.7035827383681384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC36540.32403470204148954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21280.18870986479044605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT16830.14924751054620333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT14970.13275313326658728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13520.11989461334430593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC12880.11421912868895417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT218100.0113.70121
GTGGTAT217750.0113.435976
AGTGGTA217650.0113.42655
GGTATCA218300.0113.183388
GTATCAA221350.0111.487869
TGGTATC222350.0111.1217967
CAGTGGT223600.0110.515294
AGCAGTG225200.0109.9232942
GCAGTGG228650.0108.106143
CGGTCTG5200.052.980625145
CGCTGTT8450.038.609364145
CCGCAAG15500.038.354736145
CGCAAGG7650.034.117554145
GGTGGTA7200.033.2334982
GGTCTGA7500.030.933249145
CCGACGC1204.0584366E-430.208252145
CGGAGCC4300.028.662712145
TGCGGCT8000.026.28118145
CGATAGG2550.025.589335-39
TACATGG210200.024.0123320-24
Produced by FastQC (version 0.11.5)