FastQCFastQC Report
Sun 19 Mar 2023
SRR4895818_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895818_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467313
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT137732.9472751667511927No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT31410.6721405139595946No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT17920.3834688955796222No Hit
TGGCTTCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT5730.12261589127629662No Hit
TGGCTTCATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT4700.10057498935403038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCTA351.2732926E-11144.2177
GGCTTCA467900.0144.032042
GCTTCAG146650.0143.872793
TGGCTTC469200.0143.6331
GCTTCAA123000.0143.454863
CTTCATG91500.0143.350114
GCTTCAT192100.0143.241033
CTTCAGT50800.0142.939484
CTTCAGG55450.0142.39644
CTTCAGA40500.0141.724354
CTTCAAG40900.0140.86724
TCATGTA42850.0140.178246
TCAATAG5200.0140.056886
CTTCAAT48900.0139.940614
TCATTAG5700.0139.156746
TCAGTGA5400.0138.875616
CTTCATA45600.0138.682344
TTCAGTA14800.0138.370355
TTCAGGT17650.0137.680245
TTCAGGA13950.0136.98035
Produced by FastQC (version 0.11.5)