FastQCFastQC Report
Sun 19 Mar 2023
SRR4895817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041525
Sequences flagged as poor quality0
Sequence length151
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAT383350.0143.093723
TGGAACA1051100.0142.886371
GGAACAA1061300.0141.513092
GAACAAG367700.0139.791413
AACAAGT127250.0138.820574
ACAAGTG43150.0138.522165
AACAATG138000.0138.403924
ACAATAG41750.0136.950065
ACAATTG48350.0136.29985
ACAATGT49250.0134.541055
ACAATGG45750.0134.432255
GAACAAA311950.0134.357183
AACAAGG142350.0134.174514
CAATGAG15550.0132.61156
CAAGATG10350.0132.360636
ACAAGAT32000.0132.036255
CAATAGG15900.0131.959726
ACAAGGT48200.0131.937135
ACAAGTT42150.0131.887125
ACAATGA42650.0131.017175
Produced by FastQC (version 0.11.5)