FastQCFastQC Report
Sun 19 Mar 2023
SRR4895817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041525
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG123121.1821127673363578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122691.1779842058519958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG32730.3142507381003817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC30640.2941840090252274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20170.1936583375339046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19440.18664938431626701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16780.16110991094788893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11740.11271932982885673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10520.1010057367802021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT266000.0110.226981
GGTATCA269050.0109.139128
GTGGTAT268050.0109.05946
TGGTATC270000.0108.2986157
GTATCAA271800.0108.291239
AGCAGTG271650.0107.726052
AGTGGTA271600.0107.660625
CAGTGGT275350.0106.252154
GCAGTGG276850.0105.3883743
CGGTCTG3000.053.166615145
CGCCGAA1157.1430404E-637.82605145
CGGTCGG900.00362746532.2237365
TACGCAC3500.027.7560550-54
AGCGCAT1358.0829835E-426.851828145
CGGAGCC3900.026.025616145
TCCGGCG3800.025.18370235-39
CGACCTA3850.024.85592545-49
CATGGGG62900.024.34337825-29
ACTAGCG300.001495375124.16594730-34
ATGGGAG46700.023.28691525-29
Produced by FastQC (version 0.11.5)