FastQCFastQC Report
Sun 19 Mar 2023
SRR4895816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440433
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG442400.0143.52871
GAAGAGA445750.0142.450032
AAGAGAT156300.0141.182533
AAGAGAG160000.0139.314423
AAGAGAA135150.0132.018783
GAGATGC3450.0131.643075
AGAGATT57650.0129.925034
AGATGCG1000.0129.762476
AGAGATG60150.0128.360214
AGAGATA51500.0128.08274
GAGATTG20900.0127.62395
GAGATAG16800.0127.016165
AGAGAGT60850.0126.88364
AGAGAGG64550.0125.6414264
AGAGTCG1050.0123.583296
GAGAGTG19900.0123.531555
GAGATGA16900.0122.852045
GAGAATG16500.0122.334985
GAGAGTT21400.0122.283945
GAGAGGT19900.0122.08255
Produced by FastQC (version 0.11.5)