FastQCFastQC Report
Sun 19 Mar 2023
SRR4895816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440433
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62331.4151982253827484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23770.5396961626399476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68400.0115.944258
AGTGGTA68100.0115.709885
GTGGTAT68400.0115.09646
AAGCAGT68950.0115.045511
GTATCAA69550.0114.339829
TGGTATC69250.0113.788357
CAGTGGT69900.0112.755834
AGCAGTG70750.0111.708622
GCAGTGG72400.0108.662033
CGCGCAA450.00963131848.338754
CACGCAG1209.559242E-636.24583145
CACCGGA800.002036530336.2458278
CGGTCTG1000.00607423228.99666145
GGGTACG401.0662403E-525.37495825-29
TACGCAC1450.024.99939250-54
TCAACGC68800.024.69773710-14
CAACGCA68950.024.64400710-14
TACATGG67250.024.62021320-24
GTACATG67800.024.5701820-24
AACGCAG69050.024.54532810-14
Produced by FastQC (version 0.11.5)