FastQCFastQC Report
Sun 19 Mar 2023
SRR4895815_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895815_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89323
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT57996.492168870279771No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT11711.3109725378681862No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8470.9482440133000459No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT5350.5989498785307256No Hit
TCTTCACATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT2570.2877198481913953No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGC1740.19479865208289018No Hit
TCTTCACATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT1730.1936791195996552No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTCCCCACGTACTCTGCGT1730.1936791195996552No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTT1710.1914400546331852No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCCGC1700.19032052214995018No Hit
TCTTCACATGTACTCTGCGTGGATACCACTGCTTCCATGTACTCTGCGTT1310.14665875530378514No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTC1300.14553922282055015No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCGGC1270.14218062537084514No Hit
TCTTCACATGTACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1130.12650717060555514No Hit
TCTTCACATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1020.1141923132899701No Hit
TCTTCACATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGT950.1063555859073251No Hit
TCTTCACAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG930.10411652094085509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTCA100.007192882144.235737
CATGGGT202.1763826E-6144.235737
ATGATTG100.007192882144.235739
TTGGAAG202.1763826E-6144.235739
CAAGTAG202.1763826E-6144.235737
CAGTTCA100.007192882144.235737
GAGTAAG100.007192882144.235739
ACATTCG100.007192882144.235736
AGAGTTG100.007192882144.235738
CAGATGG100.007192882144.235737
ACATGCT100.007192882144.235736
CAATGGT202.1763826E-6144.235737
ACACTAG100.007192882144.235736
TAGGATA100.007192882144.235739
CAGTAGG100.007192882144.235737
CAATATG400.0144.235737
GTAAATG100.007192882144.235739
GCATCAT100.007192882144.23573145
CAGACCT100.007192882144.235737
ACAGTTG1100.0144.235726
Produced by FastQC (version 0.11.5)