FastQCFastQC Report
Sun 19 Mar 2023
SRR4895815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89323
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1597017.878933757262967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33913.7963346506498885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10641.191182562162041No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3550.3974340315484254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG2010.2250260291302352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1960.21942836671406019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGAAGAAGATCGGAAGAGCACA1940.2171893017475902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1900.2127111718146502No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1650.18472285973377517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1640.18360332725054015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATTTCAATATTCTTAC1450.16233221006907514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACCGGTCGTCTCGTCCCTT1360.15225641771996015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1260.14106109288761012No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1220.13658296295467012No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1210.13546343047143514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1160.12986576805526012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGTCCCTTGGAGCATAAT1140.12762670308879012No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1130.12650717060555514No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1130.12650717060555514No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1110.12426810563908512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACGAGAATAGACAAATAGA1070.11978997570614512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAAAATTTTGTCAAAAC1060.11867044322291011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTTTCTATGACAAAGGCTGT1030.11531184577320511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTAGTTATCCAAGCAAAA1030.11531184577320511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAATCGAAGGTGAAGATCA1020.1141923132899701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAATGTCTCTATGAAAGTGA1000.11195324832350009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAACAATATCATCGTCGGAT980.10971418335703009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGTTACAGGTTGTCGA980.10971418335703009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTTACAATAGATGC970.1085946508737951No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGACTGACTTTTGGTAGC960.1074751183905601No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA950.1063555859073251No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC940.1052360534240901No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG930.10411652094085509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAG100.00708013145.0145
CTGGTTA406.0716775E-790.625145
TACGAAC353.4201268E-582.85714145
CCTGGGT406.6300396E-572.5145
CGTGGGC554.031419E-665.90909145
AAGCAGT132100.065.475021
AGTGGTA134200.064.396425
AGCAGTG134550.064.28282
CAGTGGT134500.064.198894
TGGTATC135650.063.814977
GTGGTAT135450.063.8021436
GTATCAA136300.063.72349
GGTATCA136400.063.6766858
GCAGTGG137000.063.0273743
CGAGCGC350.003555878862.142857145
AATGGGG502.0003013E-458.0145
TATGTGC502.0003013E-458.0145
AAGGATC400.00603292854.375145
GATGGAG553.2031274E-452.727272145
CAGTGAT450.00961063348.3333364
Produced by FastQC (version 0.11.5)