FastQCFastQC Report
Sun 19 Mar 2023
SRR4895814_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895814_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767577
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA768250.0144.070362
CGTCTAG264950.0144.065693
CGTCTAA223400.0143.8793
CGTCTAT266400.0143.76873
TCCGTCT770400.0143.668291
GTCTAGT92750.0143.346634
GTCTAGG95150.0143.292434
GTCTAAG78200.0142.910934
GTCTAAT78850.0142.830144
GTCTAGA76250.0142.688814
GTCTATG98850.0142.670014
GTCTATA79100.0141.83184
GTCTAAA67750.0140.476994
CTAGAGT7950.0139.66716
TCTAGAG25200.0139.623925
GTCTATT90600.0139.108544
TCTAGGT32050.0138.802615
TCTAAGG27550.0138.182435
TCTAGTG32500.0137.989975
TCTAGGA26300.0137.896355
Produced by FastQC (version 0.11.5)