FastQCFastQC Report
Sun 19 Mar 2023
SRR4895814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767577
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85231.1103771999421557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59100.7699553269574257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA15730.20493058025448913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT13300.17327251858771173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11080.14435033879337186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9360.12194216345721667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7710.10044594874520732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT143600.0109.5293051
GGTATCA143800.0109.053098
GTGGTAT143750.0108.536236
AGTGGTA144300.0108.223045
GTATCAA145900.0107.774579
TGGTATC145950.0106.9498757
CAGTGGT146750.0106.479534
AGCAGTG148650.0105.459992
GCAGTGG152350.0102.470423
CGGTCTG2050.042.43649145
TGCGGCT5500.039.54309145
AATAGTT7750.030.869125145
CATGGGG53550.024.53522125-29
CGACCTA2700.023.62852745-49
TACGCAC2900.023.49859650-54
TCCGGCG3100.023.38600335-39
TACATGG140200.022.98206320-24
CAACGCA143250.022.96581610-14
ACATGGG137000.022.78853620-24
GTACATG143150.022.7718320-24
Produced by FastQC (version 0.11.5)