FastQCFastQC Report
Sun 19 Mar 2023
SRR4895813_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895813_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353031
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28290.8013460574283845No Hit
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6700.18978503304242406No Hit
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT3660.10367361506496597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC353350.0144.070051
ATCAGCA353400.0144.049672
TCAGCAG118100.0143.846563
TCAGCAT129100.0143.766073
CAGCAGT42150.0143.52864
CAGCATG48800.0143.178574
TCAGCAA99300.0142.760593
CAGCATT46550.0141.889374
CAGCATA33900.0141.660464
CAGCAAG36050.0141.412644
AGCAGTA12400.0141.305375
GCATTAG4900.0141.269766
AGCATGG14500.0141.229175
AGCATGT21250.0141.158975
CAGCAAT35400.0141.157534
CAGCAGG45250.0141.025864
AGCAGGT14550.0140.743855
AGCAGTT14250.0140.67085
AGCAGTG13750.0140.542025
AGCATGA11650.0140.499255
Produced by FastQC (version 0.11.5)