FastQCFastQC Report
Sun 19 Mar 2023
SRR4895813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353031
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG129313.662851137718784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34540.978384334520198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA26010.7367624939452909No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT10390.2943084318374307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGACGCAGTGGCTCA9300.2634329563126184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACAGTGGCTCACGCTT8610.24388793052168226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCACAGTGGCTCACGCTTG8080.22887508462429645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGACGCAGTGGCTCAC7080.2005489602896063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7040.19941591531621872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6540.1852528531488736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCATGCCT5450.15437737762406134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5160.1461628015670012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGTGCGCGGTGGCTCA4940.13993105421336935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGTGCGCGGTGGCTCAC4020.11387101982545443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG3850.1090555786885571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT172600.097.549251
CATGCGT301.5979647E-596.66733145
AGCAGTG178450.094.175482
AGTGGTA178850.093.870495
GGTATCA179250.093.863228
CAGTGGT179050.093.7789154
GTGGTAT179450.093.516236
GTATCAA180900.093.207449
TGGTATC180300.093.115577
GCAGTGG185150.090.610943
AAACCGT450.00963375148.33366145
CATGCGG700.001055675741.428856145
ACTCGGC700.001055675741.428856145
GCACTTG4150.038.434145
TAGCGTT800.002034764536.2502483
GAGTCGA900.003624954732.222443145
CGATCGT200.006077177428.998556105-109
CGTAAGG304.402087E-528.998554100-104
CGTAGAA200.006078018328.99773690-94
ATGGGGG18750.026.44443525-29
Produced by FastQC (version 0.11.5)