FastQCFastQC Report
Sun 19 Mar 2023
SRR4895812_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895812_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292582
Sequences flagged as poor quality0
Sequence length151
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC151.2556113E-4144.225956
TGACGCT100.007202659144.225956
TAGGATG293200.0143.90621
AGGATGA293350.0143.832632
GGATGAT114050.0143.214283
GGATGAG86750.0143.062163
GGATGAA90500.0142.63233
GATGATG39550.0138.026614
GATGAAT35900.0137.195424
GATGATA34850.0136.983614
GATGAAG30650.0135.050064
GATGAGG29300.0134.873414
GATGAGT34250.0134.751254
ATGATGA11800.0134.44795
GATGAGA27500.0133.999024
GATGATT44650.0133.889484
GGATGAC4050.0133.542543
ATGAGTA11600.0132.414345
TGAATCG1150.0131.684566
ATGAGAG8850.0131.188575
Produced by FastQC (version 0.11.5)