FastQCFastQC Report
Sun 19 Mar 2023
SRR4895812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292582
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31451.0749123322692442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27430.9375149530729846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6830.23343883082349565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5700.1948171794573829No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4900.1674744174282765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3960.13534667204407655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3670.12543492080852547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC3270.11176353979397229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC2980.10185178855842124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT62150.0114.668811
GTGGTAT62650.0112.827876
GGTATCA62900.0112.2641758
AGTGGTA63400.0111.607515
TGGTATC63600.0111.0285647
CAGTGGT63800.0111.0214164
AGCAGTG63950.0110.8743742
GTATCAA64600.0109.646549
GCAGTGG65250.0108.5542763
TGCGGCT2150.040.471558145
GGATGAT1655.9926606E-835.1511043
GCCCCAA3100.035.086227145
ATCGCCC850.002736694234.123077145
AGAGAGT3250.031.235739145
AGTGATA1652.3790344E-630.7572154
GACGTAG353.7765349E-628.99965785-89
GTGATAT1701.0289341E-425.5879345
CATGGGG21300.025.45979325-29
CGACCTA1150.025.21709645-49
TCTCGCA1350.024.70341370-74
Produced by FastQC (version 0.11.5)