FastQCFastQC Report
Sun 19 Mar 2023
SRR4895811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879441
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274610.9536920534228691No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG117400.40771802582515143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC74970.26036303574200687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT51780.17982657050448334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC35400.12294052908186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC30900.10731249572399643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA491150.0120.167498
AAGCAGT493700.0119.8403851
GTGGTAT493450.0119.061716
AGTGGTA494650.0118.996855
TGGTATC498950.0117.954747
GTATCAA502450.0117.650489
CAGTGGT505950.0116.3615654
AGCAGTG508900.0116.014712
GCAGTGG520450.0113.050023
CGGTCTG8900.036.657345145
CCGCAAG35450.031.699612145
GTCGTAG1952.6081761E-729.7462041
TGTTCTA32800.026.3047542
CGCAAGG20850.026.079165145
CAACGCA492600.025.3427110-14
CGATAGG8650.025.31286835-39
TCAACGC493600.025.26199510-14
GTGTTCT36100.025.1060851
TACATGG489100.024.94668820-24
GTACATG495100.024.93722520-24
Produced by FastQC (version 0.11.5)