FastQCFastQC Report
Sun 19 Mar 2023
SRR4895810_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895810_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180267
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA1181850.0144.009522
CTGTCAG390000.0143.908943
GTCTGTC1183250.0143.839131
CTGTCAT423250.0143.812943
CTGTCAA351700.0143.692213
TGTCATG157300.0142.508764
TGTCAAG126300.0142.149574
TGTCAGT144250.0142.105414
GTCATAG44100.0142.079285
TGTCATA127550.0142.056644
TGTCAGA107100.0142.050424
TGTCAGG140050.0142.042454
GTCAAGT43250.0141.704095
GTCATGA41000.0141.390995
GTCAGTA42500.0141.320655
TGTCAAT127200.0141.257174
GTCAGAG38250.0140.246195
GTCAATG45550.0140.089145
GTCAGGT47800.0139.981175
GTCAGAT34650.0139.834925
Produced by FastQC (version 0.11.5)