FastQCFastQC Report
Sun 19 Mar 2023
SRR4895810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180267
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89780.7606753387157313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG74220.6288407623020893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG48340.40956834343415516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32120.27214181197983167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC26850.22749089824590538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG16710.14157813443907183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGTGCAGTGGCTCA14590.12361609703567074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12940.10963620943396707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT370600.0125.607061
GGTATCA378700.0122.904838
GTGGTAT378950.0122.3072056
TGGTATC379950.0122.061637
GTATCAA382850.0121.629389
AGCAGTG382550.0121.469752
CAGTGGT383450.0120.957734
AGTGGTA386400.0120.005345
GCAGTGG393950.0117.6602253
TAGCGAC450.00963710448.3364562
GCACGAA700.001056162641.4330021
AAATATA31450.029.50656145
CGGTATC1255.155004E-428.9994167
GCGGTAT1306.4844126E-427.8840546
CGGTCTG3900.027.884054145
GAGGTAT3700.027.4318796
CATGGGG119800.025.81669425-29
ACATGGG365950.025.69580320-24
ACCGTTC1701.0315883E-425.587719145
TCAACGC381500.025.56357610-14
Produced by FastQC (version 0.11.5)