FastQCFastQC Report
Sun 19 Mar 2023
SRR4895809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110384
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC14170.12761351028112797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG1110900.0144.146641
TCGTAGA1111100.0144.120682
CGTAGAG363400.0143.88073
CGTAGAT405450.0143.79123
CGTAGAA329900.0143.606023
GTAGATG140150.0141.491124
GTAGAGT125550.0141.288884
GTAGAGA110550.0140.565294
GTAGAGG131250.0140.537054
GTAGATA127250.0140.421344
GTAGAAT124150.0139.74574
GTAGAAG112550.0139.541034
GTAGATT145050.0138.451314
CGTAGAC15700.0138.247243
TAGATGA38200.0136.478595
TAGATAG42400.0135.884675
TAGAGTG42000.0135.461945
TAGATGG49850.0135.394275
TAGATTG49500.0135.331885
TAGAGTA38850.0135.123215
Produced by FastQC (version 0.11.5)