FastQCFastQC Report
Sun 19 Mar 2023
SRR4895809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110384
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111451.0037068257467687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG78610.7079532846294615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT17260.15544172106226314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13070.1177070274787821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11970.10780054467643627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11820.10644966065793457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC11110.10005547630369314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210900.0114.297698
GTATCAA213150.0113.261239
GTGGTAT213500.0112.561136
AAGCAGT215450.0112.3314361
AGTGGTA216050.0111.304715
TGGTATC216300.0111.109037
AGCAGTG220350.0109.5653152
CAGTGGT221950.0108.350834
GCAGTGG227000.0105.844563
GTCGTAG3700.092.099571
CGTAGAT1700.076.7653963
TCGTAGA4550.074.8907852
CGGTCTG3550.046.97014145
CGTAGAA2750.042.1821983
CGTAGAG2755.456968E-1231.6366483
CGGAGCC3503.6379788E-1226.927603145
CATGGGG76750.024.44778625-29
CAACGCA209750.024.05612810-14
TCAACGC211050.023.8873410-14
AGAGTAC210250.023.86077915-19
Produced by FastQC (version 0.11.5)