FastQCFastQC Report
Sun 19 Mar 2023
SRR4895808_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895808_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388530
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT60081.546341337863228No Hit
GTACGCAATGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC9160.2357604303399995No Hit
GTACGCAAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC8460.21774380356729212No Hit
GTACGCAAGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA7040.18119578925694285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA389050.0144.025571
TACGCAA389300.0143.933092
CGCAATA36700.0143.836494
CGCAAGT60050.0143.74914
ACGCAAT131150.0143.73463
CGCAATG50550.0143.516174
ACGCAAG147850.0143.497853
GCAATAG13850.0142.667425
CGCAATT42900.0142.548484
GCAATGT18550.0142.285685
ACGCAAA106100.0141.986513
GCAAGTT26650.0141.794085
CGCAAAT37850.0141.752614
CGCAAGG51750.0141.581794
GCAATGA12450.0141.33335
GCAAGGT17050.0140.84585
GCAAGAT12400.0140.740055
GCAATAT11350.0139.781885
GCAATTG15800.0139.208835
GCAAGTG18550.0139.17565
Produced by FastQC (version 0.11.5)