FastQCFastQC Report
Sun 19 Mar 2023
SRR4895808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388530
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG253646.528196020899286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA51381.3224204051167219No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26160.6733070805343216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18920.4869636836280338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10430.26844773891334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT9650.24837206908089465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7320.1884024399660258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC7220.1858286361413533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5510.14181659073945382No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5450.14027230844465033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTTGC5120.13177875582323115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA219250.063.980189
AAGCAGT220500.063.5270231
GTGGTAT220000.063.432556
GGTATCA220900.063.3381928
TGGTATC220700.063.2970477
AGTGGTA221600.062.974555
CAGTGGT224400.062.2290844
AGCAGTG225300.062.0126762
GCAGTGG228300.061.070763
ACGCAAT953.1313903E-853.423763
GCACTTG4400.041.189964145
CCGGACG907.477423E-540.27463145
CGTGGGA3550.036.757694145
AAATATA11350.031.297113145
TAGTATC1402.7438538E-531.0690027
CGCAATG950.004722605530.5278634
TGCGGCT2151.8058927E-830.346466145
CGGTCTG1204.0553804E-430.205973145
GTACGCA3351.8189894E-1228.1357521
CGCTGTT1050.007705682427.61689145
Produced by FastQC (version 0.11.5)