FastQCFastQC Report
Sun 19 Mar 2023
SRR4895807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326719
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC1200.0144.234014
TGCGAAT115700.0144.171683
GTGCGAA327000.0144.101682
TGCGAAG113800.0144.043883
GGTGCGA327200.0144.01361
GCGAATA35900.0143.631364
GCGAAGT42400.0143.553674
TGCGAAA92500.0143.454363
GCGAATG39950.0143.15094
CGAATAG11400.0142.96885
GCGAATT39450.0142.771564
CGAAGTA11500.0142.352695
CGAATTA11350.0142.327845
GCGAAAT33350.0142.287814
CGAATGT14500.0142.244575
CGAAGTG14150.0142.195365
CGAAGTT14000.0142.173525
CGAATTG14650.0141.772695
CGAATGA11550.0141.736455
GCGAAGA31100.0141.219474
Produced by FastQC (version 0.11.5)