FastQCFastQC Report
Sun 19 Mar 2023
SRR4895807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326719
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59471.8202185976328282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19040.5827637817206835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17340.5307313012099082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11720.3587180421095804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11550.3535147940585029No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9090.2782207340252633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7910.24210407108248982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA4070.12457187981109148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC3730.11416538370893642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT3520.10773784199878184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT94900.0104.067121
GGTATCA95350.0103.7122048
GTGGTAT95150.0103.3968356
GTATCAA96100.0103.053679
TGGTATC95800.0102.846647
CAGTGGT95750.0102.68894
AGCAGTG96850.0101.672332
AGTGGTA98150.0100.236465
GCAGTGG99850.098.472343
GGTGCGA1151.4070247E-744.1370051
TCTATAC1755.2750693E-1141.4284145
TGCGGCT1401.4095349E-841.428394145
CGCAAGG2600.039.0383145
CCGCAAG4000.038.06234145
GTGCGAA1652.377179E-630.7621542
GGGTATC950.004722784330.5261887
AAATATA10650.029.952925145
ATAGCAA3301.8189894E-1228.560484145
CGTGGGA1958.7312255E-626.025532145
CATGGGG28250.025.3043225-29
Produced by FastQC (version 0.11.5)