FastQCFastQC Report
Sun 19 Mar 2023
SRR4895806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672788
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC675350.0143.65871
GAGAACA675650.0143.594912
GAACAGT86650.0143.381734
AGAACAT232250.0143.127263
AGAACAG239000.0142.976913
GAACATG83650.0142.231284
GAACATA69350.0141.406564
GAACATT79850.0140.962984
GAACAAT67950.0139.650834
GAACAGA66350.0139.649484
GAACAGG87700.0139.609594
AGAACAA199500.0139.298343
GAACAAG74600.0138.994374
AACAGTG29050.0136.76745
ACAGTAT9250.0136.418556
AACATGA24500.0135.97315
AACATGG31800.0135.370595
AACATTG29100.0134.797875
AACAGTA27500.0134.512245
AACATGT27750.0134.33985
Produced by FastQC (version 0.11.5)