FastQCFastQC Report
Sun 19 Mar 2023
SRR4895806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672788
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85211.2665208059596784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG46530.6915997312675017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11340.16855235230117066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8840.13139354447463392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7510.11162505871091637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT7410.11013870639785489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137750.0114.6278158
AGTGGTA137800.0114.3842855
GTGGTAT137500.0114.3616946
AAGCAGT138700.0114.1125261
TGGTATC138650.0113.465457
GTATCAA140450.0112.3726049
AGCAGTG140900.0112.279332
CAGTGGT140800.0112.10164
GCAGTGG143300.0109.8423163
CCGACGC803.7324266E-545.31438145
CGGTCTG1856.184564E-1135.271736145
CACGCAG2405.2641553E-827.18863145
GGTCTGA3751.0913936E-1125.134377145
CATGGGG51650.024.59346825-29
CGACCTA2500.024.35920145-49
CAACGCA137400.024.03924210-14
AACGCAG137550.023.91815410-14
TCAACGC138300.023.89328810-14
ACATGGG131150.023.88310820-24
TACATGG135750.023.82157320-24
Produced by FastQC (version 0.11.5)