FastQCFastQC Report
Sun 19 Mar 2023
SRR4895805_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895805_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476373
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGGTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT6360.13350882606696854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA476650.0144.114442
GCTCGGT477100.0143.978521
TCGGTAT165400.0143.900073
TCGGTAG167850.0143.861563
TCGGTAA134350.0143.829533
CGGTATG60800.0143.730834
CGGTAAG49450.0143.330354
CGGTATA50150.0143.198794
CGGTAGT60200.0143.127274
CGGTAGG64250.0142.746324
CGGTAGA43300.0142.706544
CGGTATT54050.0142.070824
CGGTAAT45650.0141.678054
TCGGTAC10150.0139.942983
CGGTAAA40350.0139.559194
GGTATGA18150.0135.068245
GGTAGTA19300.0133.744725
GGTATAG18250.0133.537965
GGTAAGA13650.0132.584275
GGTAGGA17900.0132.120975
Produced by FastQC (version 0.11.5)