FastQCFastQC Report
Sun 19 Mar 2023
SRR4895805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50011.0498076087435686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29720.6238808664638844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10690.22440398595218453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9670.20299219309238767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8830.18535895191373147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8520.17885144624065596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC6380.13392866514265084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5370.11272679182069514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC5220.10957799875307794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101700.0107.0242848
GTGGTAT101800.0106.705466
AAGCAGT102700.0106.524671
TGGTATC102400.0106.292667
GTATCAA102350.0106.180619
AGTGGTA103250.0105.277155
AGCAGTG104450.0104.692482
CAGTGGT105250.0103.4144444
GCAGTGG107550.0101.337733
CGGTCTG1400.072.49889145
CTCGGTA553.214196E-452.737542
GCTCGGT1051.8524281E-434.5232851
GCCCCAA6350.030.826302145
CCGCAAG5450.030.595865145
CGACCTA2100.026.23769845-49
CGGCGGT2350.024.06346135-39
CATGGGG35000.023.94535325-29
TACCTCG1350.023.6292735-39
TCCGGCG2300.023.32573135-39
TACGCAC2450.023.08128250-54
Produced by FastQC (version 0.11.5)