FastQCFastQC Report
Sun 19 Mar 2023
SRR4895804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500474
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG500700.0144.13631
CTAACGA500750.0144.121922
TAACGAT174650.0144.043173
TAACGAG175900.0143.921373
TAACGAA141450.0143.749543
AACGAGT61150.0143.500824
CGATTAG6550.0143.10756
AACGATT58750.0142.858294
AACGATG64750.0142.760654
ACGAGTG19150.0142.702225
ACGAGTA18950.0142.686335
AACGATA51300.0142.662224
ACGATAG18500.0142.259545
ACGATGT21300.0142.17725
ACGATGA17250.0142.118335
CGATAAG6600.0142.023336
CGATTGA5750.0141.700336
ACGAAGT17150.0141.685715
ACGATTG20950.0141.454935
ACGAGAT15400.0141.399065
Produced by FastQC (version 0.11.5)