FastQCFastQC Report
Sun 19 Mar 2023
SRR4895804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500474
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG74091.4803965840383317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37500.7492896733896266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG37090.7410974396272334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18680.37324616263781935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13170.2631505332944369No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6560.13107574019829202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6030.12048577948105196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT219500.0119.344381
GTATCAA221050.0118.003749
GGTATCA221700.0117.755878
TGGTATC222750.0117.0055167
GTGGTAT223000.0116.8418356
AGCAGTG225100.0116.375342
CAGTGGT224700.0116.163044
AGTGGTA225500.0115.578615
GCAGTGG227800.0114.5822453
TTAGCGA259.3850936E-486.99743145
CTAACGA1000.072.505092
GCGTAAT350.003565881662.141018145
GCTAACG1602.0008883E-1145.3156851
AAATATA16350.039.02025145
TAACGAT1001.3898815E-436.2525443
CCGCAAG3050.030.900724145
CACGCAG2705.5515557E-926.851055145
CATGGGG47650.025.77620725-29
AAACCGC7750.025.63149885-89
GAGGTAT2301.1015582E-625.2166446
Produced by FastQC (version 0.11.5)