FastQCFastQC Report
Sun 19 Mar 2023
SRR4895803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905890
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA906350.0144.140341
CGAGTAA906750.0144.076752
GAGTAAG325850.0143.157673
GAGTAAT319750.0142.979553
GAGTAAA256300.0141.997253
AGTAAGT121300.0139.999134
GAGTAAC13450.0139.930833
AGTAAGA90200.0138.703744
AGTAATG121850.0138.420334
AGTAAGG121500.0138.166234
AGTAAAG101000.0136.152164
GTAAGTG41850.0136.121525
AGTAATA94550.0136.059394
AGTAATT114500.0135.843814
GTAATGG46600.0135.244845
GTAATAG37950.0135.099275
GTAATTG44050.0134.72535
GTAAGTA37500.0134.412955
GTAAGAG32750.0134.091485
AGTAAAT95950.0133.69844
Produced by FastQC (version 0.11.5)