FastQCFastQC Report
Sun 19 Mar 2023
SRR4895802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388705
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG86512.22559524575192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40081.0311161420614605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG32860.8453711683667563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15150.389755727351076No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA14700.3781788245584698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA11750.3022857951402735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTAGACGCAAACAAAATA9780.25160468735930847No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA9720.25006110032029427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT9590.24671666173576362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA8970.23076626233261727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCACTGTATTTTAATATTT8620.22176200460503467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGTTATTCTCTCATGAA8600.22124747559202992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGAAGAAATGCAAACTAT8350.21481586292947094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATATTAAAGTTGCTGCAGTT8280.2130150113839544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGATGGGTGACCACCT8050.2070979277344001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTTAATATTTGTTATTCT6730.17313901287608857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA6470.16645013570702719No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGTATTTTAATATTTGTT6420.1651638131745154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGATAGCCCTTATGAAACT6410.16490654866801302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCATTGCTTTCGTGTAAAT6300.16207663909648706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCAATAGGCAGCTTTCTT5560.1430390656153124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGCTTTCTTAACTATC5140.13223395634221324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAGAAGATGGGTGACCA5100.1312048983162038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTTAGTTTGTGGCTTCAT4990.12837498874467784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTCTTCGAAAGGCTCACT4540.11679808595207163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTCTCATGAATAGAAAT4390.11293911835453621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTGATGGCCGGTGAGCTG3980.10239127358793944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT274150.0122.889371
GGTATCA280350.0120.1353768
AGTGGTA279350.0120.0982365
GTGGTAT279850.0119.909576
AGCAGTG280750.0119.757612
GTATCAA281800.0119.6819469
GCAGTGG281300.0119.136823
TGGTATC282150.0119.034897
CAGTGGT282100.0118.953184
CGGTCTG502.303841E-672.49864145
AGGGGTA1550.051.4572755
GGGTATC1851.8189894E-1243.1128547
CAGGGGT1851.8189894E-1243.1128544
CTCGGCG5000.039.14927145
GGGGTAT2109.094947E-1237.9803666
CGTATTT1051.8518402E-434.523167145
TATGCAT2154.8567017E-1033.720303145
GATATCA2850.033.0738148
AAGTATA4200.032.79701145
GAGGTAT1153.1565325E-431.5252086
Produced by FastQC (version 0.11.5)