FastQCFastQC Report
Sun 19 Mar 2023
SRR4895801_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895801_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198109
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41960.3502185527360199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC1199400.0144.054081
ATAGACA1200650.0143.904082
TAGACAG401950.0143.636323
TAGACAT431000.0143.624833
AGACATG161100.0143.091424
AGACAGT143200.0142.90124
TAGACAA354400.0142.826873
GACATAG44500.0141.779855
GACAATG45700.0141.68595
GACAGGT48950.0141.26435
GACATGA42500.0141.156515
AGACATT143550.0141.146354
GACATTG48900.0141.113835
GACAGAT39350.0140.912055
GACATGT59200.0140.799995
AGACAAT127250.0140.753864
GACAGTG46150.0140.616845
GACAGTA43450.0140.393545
AGACATA129400.0140.365524
GACAGTT48850.0139.929825
Produced by FastQC (version 0.11.5)