FastQCFastQC Report
Sun 19 Mar 2023
SRR4895801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198109
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG138811.1585757222423003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107770.899500796672089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG28570.23845910513984955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24550.2049062314029859No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15200.12686658726376315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT250050.0103.146271
GGTATCA251400.0102.208898
GTGGTAT251250.0102.063666
TGGTATC253050.0101.3991857
GTATCAA255350.0100.7339
AGCAGTG256300.0100.513632
AGTGGTA255900.0100.237375
CAGTGGT257250.099.776034
GCAGTGG266650.096.31313
GATAGAC4800.087.615971
ATAGACA7100.058.2096372
CGGTCTG4450.048.876873145
TAGACAG4800.033.2322543
AGCGGTA900.003628347532.2225275
AGCGCAT1701.0313933E-425.588476145
CATGGGG86000.024.49848725-29
CATGCGA2151.8659995E-523.604876145
GGTCTGA7500.023.20022145
CGATAGG452.6545462E-522.55539135-39
ACATGGG230500.021.94024320-24
Produced by FastQC (version 0.11.5)