FastQCFastQC Report
Sun 19 Mar 2023
SRR4895800_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895800_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574555
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGA574950.0144.124881
AGCTGAA575250.0144.049712
GCTGAAT203650.0143.871123
GCTGAAG197300.0143.567323
CTGAATG71700.0143.52124
GCTGAAA167450.0143.450043
CTGAAGT70100.0142.785034
CTGAAAT59900.0142.780564
CTGAAAG61100.0142.454864
CTGAAGA57600.0142.22214
CTGAATT67650.0142.093294
CTGAAGG70200.0141.65714
CTGAATA64300.0141.533624
TGAATAG22800.0137.583285
GCTGAAC9000.0135.411473
TGAATGG25200.0135.354235
TGAAGTG23300.0133.392855
TGAATGA22250.0132.881675
CTGAAAA50050.0132.266484
TGAAGTA24950.0132.086015
Produced by FastQC (version 0.11.5)