FastQCFastQC Report
Sun 19 Mar 2023
SRR4895800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574555
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53240.9266301746569083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36310.631967348643733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20480.3564497741730557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13720.23879350105734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT106100.0119.114071
GGTATCA107350.0118.0442358
GTGGTAT107850.0116.488716
TGGTATC108350.0116.352597
AGCAGTG109300.0115.494062
GTATCAA110450.0114.927999
AGTGGTA110150.0114.1979145
CAGTGGT110700.0113.640434
GCAGTGG111450.0112.6154563
CGGTCTG1307.346898E-944.612038145
CGTACCA1209.561689E-636.24728145
TGCGGCT3050.035.653065145
AAAACGG950.00472737330.524025145
GCCCCAA5400.025.507345145
CATGGGG41450.024.87373425-29
ACATGGG103200.024.48969320-24
TACATGG105950.024.46990220-24
GTACATG107000.024.39239120-24
CAACGCA108950.024.32676710-14
GCAGAGT107900.024.21412315-19
Produced by FastQC (version 0.11.5)