FastQCFastQC Report
Sun 19 Mar 2023
SRR4895799_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895799_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902746
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT903050.0144.152921
ACTAGTA903400.0144.097082
CTAGTAG305800.0143.996613
CTAGTAT311900.0143.561523
CTAGTAA272700.0143.521353
TAGTAGA97350.0136.13554
CTAGTAC16400.0135.855263
TAGTAGG108400.0135.295534
TAGTATG119850.0135.124334
TAGTAGT117200.0134.857394
TAGTAAG97400.0134.066824
AGTAGTG35550.0133.66195
AGTAGTA39600.0133.101825
AGTATGA34900.0132.432455
TAGTAAT105150.0132.139984
TAGTATA103550.0131.95354
AGTAATG35250.0130.503885
AGTATGG43950.0129.93565
AGTAGAG31250.0129.90335
AGTAGGT38700.0129.489825
Produced by FastQC (version 0.11.5)