FastQCFastQC Report
Sun 19 Mar 2023
SRR4895799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902746
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80590.8927206545362705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75690.8384418208444014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14060.15574702075666907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11600.12849683078075116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT264750.0120.392261
GGTATCA267500.0118.863768
GTGGTAT267400.0118.582896
AGTGGTA268550.0118.081635
TGGTATC268800.0118.019217
CAGTGGT269050.0117.9495544
AGCAGTG270100.0117.920532
GTATCAA270150.0117.7246259
GCAGTGG275650.0114.9939353
ACTAGTA5300.039.6711582
GACTAGT5750.039.090451
CGGTCTG2303.4877303E-828.368958145
CATGGGG75400.025.05826825-29
ATCGGCG1450.001226503524.999466145
ACATGGG255450.024.88930520-24
CAACGCA267550.024.80878610-14
AGAGTAC267250.024.75525515-19
TACATGG264200.024.7290520-24
TCAACGC269550.024.63008710-14
ATCAACG268850.024.5917510-14
Produced by FastQC (version 0.11.5)