FastQCFastQC Report
Sun 19 Mar 2023
SRR4895798_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895798_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396121
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT6350.1603045534066611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCAG400.0144.20217
TGACGAC100.0072071766144.20216
GAATCTG396700.0143.9841
AATCTGA396700.0143.9842
ATCTGAG143700.0143.700353
ATCTGAT135200.0143.508823
TCTGAAT40800.0143.318514
ATCTGAA112800.0143.307243
CTGAATG14100.0143.17945
TCTGATA40650.0142.605764
TCTGAGT53250.0142.577294
TCTGATG51100.0142.226724
CTGAGTG17400.0142.130235
TCTGAGA42300.0141.986244
TCTGAGG48100.0141.354034
CTGAGAG12150.0141.234975
CTGATTG14050.0141.123055
TCTGAAG37100.0141.092624
CTGATGA13800.0141.067285
CTGATAG13750.0141.055865
Produced by FastQC (version 0.11.5)