FastQCFastQC Report
Sun 19 Mar 2023
SRR4895798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396121
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59311.4972697736297746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49271.2438118655663295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11570.2920824697504046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9270.2340194031621651No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8760.221144549266512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT155000.0112.375291
GGTATCA156650.0111.394978
GTATCAA157350.0111.0836949
GTGGTAT156050.0111.033496
TGGTATC156750.0110.815157
AGTGGTA157900.0109.792365
CAGTGGT157850.0109.78124
AGCAGTG158850.0109.455262
GCAGTGG159600.0108.6683353
GTGATAT2200.039.5487945
CGATAGG200.006074868629.00098435-39
AGGGGTA1658.4011495E-526.3658645
TGATATC3357.4578566E-1125.9690656
CATGGGG31600.024.87235325-29
AGTGATA3501.2914825E-1024.8592434
TAGTATC1751.2542108E-424.8561047
TACGCAC601.710032E-824.16565750-54
ATGGGAT24100.024.06842425-29
ATGGGAG22750.023.77563525-29
ACATGGG147300.023.6487320-24
Produced by FastQC (version 0.11.5)