FastQCFastQC Report
Sun 19 Mar 2023
SRR4895797_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895797_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514959
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT515200.0144.148571
GGCATAG169200.0144.090713
TGGCATA515500.0144.06472
GCATAGA51050.0143.51234
GGCATAT181900.0143.5053
GCATAGT59600.0143.492634
GCATAGG57600.0143.467424
GCATAAG56100.0143.447344
GGCATAA156700.0143.298233
GCATATG61250.0142.805824
GCATAAT54200.0142.355964
GCATATA57450.0142.08484
CATATGG21850.0141.908425
GCATATT63850.0141.508124
CATATAG20350.0140.675115
CATAAGT18150.0140.24565
CATAGGT21450.0140.184485
CATAGTG18850.0140.01065
CATAGGA16250.0139.781075
GCATAAA47000.0139.615844
Produced by FastQC (version 0.11.5)