FastQCFastQC Report
Sun 19 Mar 2023
SRR4895797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514959
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54491.058142492897493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30370.589755689287885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12480.24234939092238414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10250.19904497251237474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9260.17982014102093563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8250.16020692909532605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC6110.11865022263908388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG5870.11398965742903805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT5650.10971747265316267No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5400.10486271722603159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT118100.0109.79821
GTGGTAT119950.0107.418846
GGTATCA120900.0106.874648
GTATCAA120950.0106.8097159
TGGTATC121600.0106.080537
AGCAGTG122850.0105.4347842
AGTGGTA122450.0104.9990545
CAGTGGT123600.0104.1495744
GCAGTGG128250.0100.373393
TGTTGCG350.003565095462.144733145
CGGTCTG1354.9639675E-737.593723145
CGAGCCG1051.8521423E-434.52485145
CCGGAGC2252.8107024E-829.000875145
AGAGAGT6900.025.218151145
CATGGGG41000.024.61441825-29
CGACCTA1500.024.16504745-49
ATGGGGG17950.023.02208325-29
ACATGGG111050.022.99394420-24
TACATGG115550.022.81374420-24
TGCGATA703.5070116E-922.7859630-34
Produced by FastQC (version 0.11.5)